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Laboratory for Functional Genome Analysis

 

 

 

 

 


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Publications

2012

2D DIGE Saturation Labeling for Minute Sample Amounts. Arnold GJ, Fröhlich T 2012 Methods Mol Biol.  854:89-112.

Vitrification at the pre-antral stage transiently alters inner mitochondrial membrane potential but proteome of in vitro grown and matured mouse oocytes appears unaffected. Demant M, Trapphoff T, Fröhlich T, Arnold GJ, Eichenlaub-Ritter U. 2012 Hum Reprod.  in press

A new strategy for the development of monoclonal antibodies for the determination of human procalcitonin in serum samples. Kremmer E, Meyer K, Grässer FA, Flatley A, Kösters M, Luppa PB, Krämer PM. 2012 Anal Bioanal Chem., 402(2):989-95.

2011

First inducible transgene expression in porcine large animal models. Klymiuk N, Böcker W, Schönitzer V, Bähr A, Radic T, Fröhlich T, Wünsch A, Keßler B, Kurome M, Schilling E, Herbach N, Wanke R, Nagashima H, Mutschler W, Arnold GJ, Schwinzer R, Schieker M, Wolf E. 2011 FASEB J.  in press

Human tryptase cleaves pro-nerve growth factor (PRONGF): hints of a local, mast cell-dependent regulation of NGF/PRONGF action. Spinnler K., Frohlich T., Arnold G.J., Kunz L., Mayerhofer A.. 2011 J Biol Chem., 286(36):31707-13.

Quantifying Attomole Amounts of Proteins from Complex Samples by Nano-LC and Selected Reaction Monitoring (SRM), Fröhlich T, Arnold GJ
2011 Methods Mol Biol., 790:141-64.

The Ecoresponsive Genome of Daphnia pulex. Colbourne JK., Pfrender ME, Gilbert D, Thomas WK, Tucker A, Oakley TH, Tokishita S, Aerts A, Arnold GJ, Basu MK, Bauer DJ, Cáceres CE, Carmel L, Casola C, Choi J-H, Detter JC, Dong Q, Dusheyko S, Eads BD, Frohlich T,Geiler-Samerotte KA, Gerlach D, Hatcher P, Jogdeo S, Krijgsveld J, Kriventseva EV, Kültz D, Laforsch C, Lindquist E, Lopez J, Manak JR, Muller J, Pangilinan J, Patwardhan RP, Pitluck S, Pritham EJ, Rechtsteiner A, Rho M, Rogozin IB, Sakarya O, Salamov A, Schaack S, Shapiro H, Shiga Y, Skalitzky C, Smith Z, Souvorov A, Sung W, Tang Z, Tsuchiya D, Tu H, Vos H, Wang M, Wolf YI, Yamagata H, Yamada T, Ye Y, Shaw JR, Andrews J, Crease TJ, Tang H, Lucas SM, Robertson HM, Bork P, Koonin EV, Zdobnov EM, Grigoriev IV, Lynch M, Boore JL., 2011 Science 331 555-561.

Proteomic characterization of archaeal ribosomes reveals the presence of novel archaeal-specific ribosomal proteins. Márquez V*, Fröhlich T*, Armache JP, Sohmen D, Dönhöfer A, Mikolajka A, Berninghausen O, Thomm M, Beckmann R, Arnold GJ, Wilson DN. 2011 J Mol Biol. 405(5):1215-32. * Contributed equally

Bovine endometrial metallopeptidases MMP14 and MMP2 and the metallopeptidase inhibitor TIMP2 participate in maternal preparation of pregnancy. Ulbrich SE, Meyer SU, Zitta K, Hiendleder S, Sinowatz F, Bauersachs S, Büttner M, Frohlich T, Arnold GJ, Reichenbach HD, Wolf E, Meyer HH. 2011 Mol Cell Endocrinol 332(1-2):48-57.

Dynamic proteome signatures in gametes, embryos and their maternal environment. Arnold G.J., Frohlich T. 2011, Reproduction, Fertility and Development, 23, 1, 81-93

2010

Identification of novel downstream targets of platelet glycoprotein VI activation by differential proteome analysis: implications for thrombus formation. Schulz C, Leuschen NV, Frohlich T, Lorenz M, Pfeiler S, Gleissner CA, Kremmer E, Kessler M, Khandoga AG, Engelmann B, Ley K, Massberg S, Arnold GJ. 2010 BLOOD 115 20

Creating new knowledge for ruminant reproduction from rapidly expanding and evolving scientific databases. Bauersachs S, Blum H, Krebs S, Fröhlich T, Arnold GJ, Wolf E. Soc Reprod Fertil Suppl. 2010;67:29-40.

In vivo chemoresistance of prostate cancer in metronomic cyclophosphamide therapy. Thoenes L, Hoehn M, Kashirin R, Ogris M, Arnold GJ, Wagner E, Guenther M. J Proteomics. 2010 May 7;73(7):1342-54.

2009

A Newcomer's Guide to Nano-Liquid-Chromatography of Peptides. Frohlich T, Arnold GJ. 2009: Methods Mol Biol, 564 123-141.

LC-MS/MS-based proteome profiling in Daphnia pulex and Daphnia longicephala: the Daphnia pulex genome database as a key for high throughput proteomics in Daphnia. Frohlich T; Arnold GJ; Fritsch R; Mayr T; Laforsch C, 2009 BMC Genomics, 10 171.

Evidence for Estrogen-Dependent Uterine Serpin (SERPINA14) Expression During Estrus in the Bovine Endometrial Glandular Epithelium and Lumen. Ulbrich SE; Frohlich T; Schulke K; Englberger E; Waldschmitt N; Arnold GJ; Reichenbach HD; Reichenbach M; Wolf E; Meyer HH; Bauersachs S., 2009 Biol Reprod, 3 3.

Highly sensitive saturation labeling reveals changes in abundance of cell cycle-associated proteins and redox enzyme variants during oocyte maturation in vitro. Berendt FJ*; Frohlich T*; Bolbrinker P; Boelhauve M; Gungor T; Habermann FA; Wolf E; Arnold GJ, 2009 Proteomics, 9 550-564. * contributed equally

2008

The brain proteome profile is highly conserved between Prnp-/- and Prnp+/+ mice. Crecelius A. C., Götz A, Arzberger T, Fröhlich T, Arnold GJ, Ferrer I, Kretzschmar HA. 200: Neuroreport, 19 1027-1031.

Single chain Fv antibodies directed against the 37 kDa/67 kDa laminin receptor as therapeutic tools in prion diseases. Zuber C.; Knackmuss S.; Rey C.; Reusch U.; Rottgen P.; Frohlich T.; Arnold G. J.; Pace C.; Mitteregger G.; Kretzschmar H. A.; Little M.; Weiss S., 2008 Molecular Immunology, 45 144-151.

Assessing quantitative post-mortem changes in the gray matter of the human frontal cortex proteome by 2-D DIGE. Crecelius A; Gotz A; Arzberger T; Frohlich T; Arnold GJ; Ferrer I; Kretzschmar H. A., 2008 Proteomics, 8 1276-1291.

2007


Dual specificities of the glyoxysomal/peroxisomal processing protease Deg15 in higher plants. Helm M; Luck C; Prestele J; Hierl G; Huesgen PF; Froehlich T; Arnold GJ; Adamska I.; Gorg A.; Lottspeich F.; Gietl C., 2007 Proceedings of the National Academy of Sciences of the United States of America, 104 11501-11506.

2006


Proteome research based on modern liquid chromatography - tandem mass spectrometry: separation, identification and quantification. Frohlich T; Arnold GJ, 2006 Journal of Neural Transmission, 113 973-994.

Analysis of the HUPO Brain Proteome reference samples using 2-d DIGE and 2-d LC-MS/MS. Frohlich T; Helmstetter D.; Zobawa M.; Crecelius A. C.; Arzberger T.; Kretzschmar H. A.; Arnold GJ, 2006 Proteomics, 6 4950-4966.

Methods for transcriptome and proteome research: Applications for studying biology of reproduction in bovine. Wolf E; Hiendleder S; Bauersachs S; Frohlich T; Sinowatz F; Blum H; Arnold GJ, 2006 Berliner und Munchener Tierarztliche Wochenschrift, 119 7-16.

HUPO Brain Proteome Project: summary of the pilot phase and introduction of a comprehensive data reprocessing strategy. Hamacher M.; Apweiler R.; Arnold GJ; Becker A.; Bluggel M.; Carrette O.; Colvis C.; Dunn M. J.; Frohlich T; Fountoulakis M.; van Hall A.; Herberg F.; Ji J.; Kretzschmar H.; Lewczuk P.; Lubec G.; Marcus K.; Martens L.; Palacios Bustamante N.; Park Y. M.; Pennington S. R.; Robben J.; Stuhler K.; Reidegeld K. A.; Riederer P.; Rossier J.; Sanchez J. C.; Schrader M.; Stephan C.; Tagle D.; Thiele H.; Wang J.; Wiltfang J.; Yoo J. S.; Zhang C.; Klose J.; Meyer H. E., 2006 Proteomics, 6 4890-4898.

Functional genome research - new strategies to improve fertility in cattle. Wolf E; Arnold GJ; Bauersachs S; Blum H; Frohlich T; Hiendleder S.; Mitko K.; Reichenbach H. D., 2006 Zuchtungskunde, 78 428-439.

Before 2006

DRO1, a gene down-regulated by oncogenes, mediates growth inhibition in colon and pancreatic cancer cells. Bommer G. T.; Jager C. J.; Durr E. M.; Baehs S.; Eichhorst S. T.; Brabletz T.; Hu G.; Frohlich T; Arnold GJ; Kress D. C.; Goke B.; Fearon E. R.; Kolligs F. T., 2005 Journal of Biological Chemistry, 280 7962-7975.

Holistic differential analysis of embryo-induced alterations in the proteome of bovine endometrium in the preattachment period. Berendt FJ *; Frohlich T *; Schmidt S. E. M.; Reichenbach H. D.; Wolf E.; Arnold GJ, 2005 Proteomics, 5 2551-2560. * contributed equally

Functional genomics: tools for improving farm animal health and welfare. Hiendleder S; Bauersachs S; Boulesteix A; Blum H; Arnold GJ; Frohlich T; Wolf E., 2005 Revue Scientifique et Technique-Office International des Epizooties, 24 355-377.

Protein stoichiometry of a multiprotein complex, the human spliceosomal U1 small nuclear ribonucleoprotein - Absolute quantification using isotope-coded tags and mass spectrometry. Hochleitner EO; Kastner B.; Frohlich T; Schmidt A.; Luhrmann R.; Arnold GJ; Lottspeich F., 2005: Journal of Biological Chemistry, 280 2536-2542.

Detection of wild type and deleted latent membrane protein 1 (LMP1) of Epstein-Barr virus in clinical biopsy material. Nicholls J.; Hahn P.; Kremmer E.; Frohlich T; Arnold GJ; Sham J.; Kwong D.; Grasser F. A., 2004 Journal of Virological Methods, 116 79-88.

Embryo-maternal communication in bovine - Strategies for deciphering a complex cross-talk. Wolf E; Arnold GJ; Bauersachs S.; Beier H. M.; Blum H.; Einspanier R.; Frohlich T; Herrler A.; Hiendleder S.; Kolle S.; Prelle K.; Reichenbach H. D.; Stojkovic M.; Wenigerkind H.; Sinowatz F., 2003 Reproduction in Domestic Animals, 38 276-289.

Overexpression of insulin-like growth factor-binding protein-2 in transgenic mice reduces postnatal body weight gain. Hoeflich A.; Wu M. Y.; Mohan S.; Foll J.; Wanke R.; Froehlich T; Arnold GJ; Lahm H.; Kolb H. J.; Wolf E., 1999 Endocrinology, 140 5488-5496.