Proteomics
LAFUGAs proteomics group
has
state-of-the-art equipment
for gel-based as well as LC-MS-based
proteomics.
LC-MS-based proteomics:
Surveyor 2-dimensional-HPLC (Proteome-X-System)
Ettan MDLC 2-dimensional nanoflow HPLC (GE Healthcare)
nanoACQUITY UPLC System (Waters)
Mass Spectrometry:
LTQ
Linear
Ion Trap ESI mass spectrometer (Thermo-Scientific)
LTQ Orbitrap XL
ESI mass spectrometer (Thermo-Scientific)
4800 MALDI TOF/TOF™ Analyzer (AB SCIEX)
5500 Hybrid Triple Quadrupole Linear Ion Trap (AB SCIEX)
Prefractionation:
Free-Flow-Electrophoresis (Tecan)
Mutidimensional HPLC BioCAD Vision (Applied Biosystems)
3100 OFFGEL Fractionator (Agilent)
Sample Preparation:
Robotic system Biomek 2000 (Beckman)
PROTEINEER spII Automated Spot Picker (Bruker Daltonics)
DigestPro MS digester (Intavis)
Gel-based separation:
Several electrophoresis systems (Multiphor, IPGphor, Ettan DALT)
Typhoon fluorescence scanner with 3 Lasers for 2D-DIGE (GE Healthcare)
Proteomeweaver 2D-gel analysis software (Definiens)
DeCyder 2-D Differential Analysis Software (Amersham Biosciences)
Peptide Synthesis:
433A peptide synthesizer (Applied Biosystems)
Multiple peptide synthesizer (Abimed)
SYRO spot synthesizer (Multisyntech)